基于运脾法拆方研究异功散治疗慢性病贫血的机制

石岭; 季玉婷; 郑秦; 姜一陵; 胡洁; 张瑞峰; 吴志豪; 夏黎; 张爱萍; 徐海涛; 罗梅宏

doi:10.3969/j.issn.1006-2157.2024.09.009

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基于运脾法拆方研究异功散治疗慢性病贫血的机制

实验研究 | 更新时间：2024-10-09

- 基于运脾法拆方研究异功散治疗慢性病贫血的机制
- Study on the mechanism of Yigong Powder in the treatment of anemia of chronic disease by means of formula components analysis based on activating spleen method
- 北京中医药大学学报 2024年47卷第9期页码：1247-1255
- 作者机构：
  
  1.上海市宝山区中西医结合医院/上海中医药大学附属宝山医院　上海　201999
  2.上海市嘉定区华亭镇社区卫生服务中心
  3.上海中医药大学研究生院
- 作者简介：
  
  石岭，男，硕士，主治医师
  #罗梅宏，女，博士，主任医师，博士生导师，主要研究方向：血液病的中医药基础与临床研究，E-mail：lmh021009@163.com
- 基金信息：
  
  国家自然科学基金项目(81774271;81804021;82104778);上海中医药大学预算内项目(2019LK100)
- DOI：10.3969/j.issn.1006-2157.2024.09.009
  中图分类号： R285.5
- 纸质出版日期：2024-09-30，
  
  网络出版日期：2024-08-20，
  
  收稿日期：2024-05-15，
- 稿件说明：
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石岭, 季玉婷, 郑秦, 等. 基于运脾法拆方研究异功散治疗慢性病贫血的机制[J]. 北京中医药大学学报, 2024,47(9):1247-1255.

SHI Ling, JI Yuting, ZHENG Qin, et al. Study on the mechanism of Yigong Powder in the treatment of anemia of chronic disease by means of formula components analysis based on activating spleen method[J]. Journal of Beijing University of Traditional Chinese Medicine, 2024,47(9):1247-1255.
石岭, 季玉婷, 郑秦, 等. 基于运脾法拆方研究异功散治疗慢性病贫血的机制[J]. 北京中医药大学学报, 2024,47(9):1247-1255. DOI： 10.3969/j.issn.1006-2157.2024.09.009.

SHI Ling, JI Yuting, ZHENG Qin, et al. Study on the mechanism of Yigong Powder in the treatment of anemia of chronic disease by means of formula components analysis based on activating spleen method[J]. Journal of Beijing University of Traditional Chinese Medicine, 2024,47(9):1247-1255. DOI： 10.3969/j.issn.1006-2157.2024.09.009.

摘要

目的

观察陈皮在异功散改善慢性病贫血铁代谢、促进红细胞生成中的作用。

方法

将异功散进行拆方分为陈皮单药和陈皮缺味方，采用2×2析因设计方法，将32只酵母糖诱导的全身炎症反应模型小鼠按随机数字表法分为模型组、陈皮组、陈皮缺味方组和异功散全方组，每组8只。陈皮组(3.083 g/kg)、陈皮缺味方组(12.33 g/kg)和异功散全方组(15.413 g/kg)小鼠分别灌胃相应药物，模型组小鼠灌胃等量生理盐水，连续7 d。给药结束后，记录各组小鼠体质量，采用全自动细胞计数仪检测各组小鼠血红蛋白含量，比色法检测血清铁含量，酶联免疫吸附测定(ELISA)法检测血清铁蛋白含量，计算脾指数，Luminex法检测肝脏组织炎症因子白细胞介素－1β(IL-1β)、白细胞介素－6(IL-6)、肿瘤坏死因子－α(TNF-α)、γ干扰素(IFN-γ)、白细胞介素－4(IL-4)和白细胞介素－10(IL-10)水平，实时荧光PCR法检测肝脏组织铁调素基因(HAMP)和膜铁转运蛋白(Fpn)的mRNA表达。

结果

陈皮组和陈皮缺味方组在调节血红蛋白、血清铁、血清铁蛋白含量，改善脾指数，调节HAMP、Fpn mRNA及IL-1β、IFN-γ表达方面均呈现交互作用(均

0.05)。与模型组比较，陈皮组血红蛋白、血清铁含量及Fpn mRNA表达水平升高，铁蛋白含量、脾指数、HAMP mRNA及IL-1β、IL-6、TNF-α、IFN-γ水平降低(均

0.05)；陈皮缺味方组血清铁含量、Fpn mRNA表达水平升高，脾指数、铁蛋白含量、HAMP mRNA及IL-1β、IFN-γ、IL-4水平降低(均

0.05)。

结论

陈皮对促炎症因子IL-6、TNF-α的抑制作用及对Fpn的上调作用可能在异功散全方改善慢性病贫血及铁代谢中发挥了关键作用。

Abstract

Objective

To observe the role of dried tangerine peel in

Yigong

Powder improves iron metabolism and promotes red blood cell generation in anemia of chronic disease (ACD).

Methods

With a two-by-two factorial design

the

Yigong

Powder was divided into dried tangerine peel and

Chenpi

absent Decoction. According to the random number table method

32 zymosan-induced generalized inflammation (ZIGI) mice were randomly divided into the model group

the dried tangerine peel group

the

Chenpi

absent Decoction group

and the

Yigong

Powder group. The dried tangerine peel group

Chenpi

absent Decoction group and the

Yigong

Powder group were given dried tangerine peel(3.083 g/kg)

Chenpi

absent Decoction(12.33 g/kg)

and

Yigong

Powder(15.413 g/kg)by gavage to the corresponding group of mice. The model group was given an equal amount of physiological saline by gavage

and treated continuously for 7 days. After the completion of administration

the body weight of each group of mice was recorded. The hemoglobin

content of each group of mice was detected using a fully automatic cell counter

the serum iron content was detected using colorimetry

the serum ferritin content was detected using enzyme-linked immunosorbent assay (ELISA)

and the spleen index was calculated. The liver tissue inflammatory factors interleukin-1β (IL-1β)

interleukin-6 (IL-6)

tumor necrosis factor-α (TNF-α)

interferon-γ (IFN-γ)

interleukin-4 (IL-4)

and interleukin-10 (IL-10) levels were detected using Luminex method. The mRNA expressions of liver tissue hepcidin gene (HAMP) and membrane iron transporter (Fpn) were detected using real-time fluorescence PCR method.

Results

Dried tangerine peel and

Chenpi

absent Decoction both showed interactive effects in regulating hemoglobin

serum iron

serum ferritin content

improving spleen index

and regulating the mRNA expressions of HAMP

Fpn

as well as IL-1β and IFN-γ (

0.05). Compared with the model group

dried tangerine peel significantly increased hemoglobin

serum iron content

and Fpn mRNA expression in ZIGI model mice

while decreasing ferritin content

spleen index

HAMP mRNA expression

and the levels of IL-1β

IL-6

TNF-α

and IFN-γ (

0.05).

Chenpi

absent Decoction significantly increased serum iron content and Fpn mRNA expression in ZIGI model mice

while reducing spleen index

ferritin content

HAMP mRNA expression

and the levels of IL-1β and IFN-γ、IL-4 (

0.05).

Conclusion

The effects of dried tangerine peel on inflammatory factors (IL-6 and TNF-α) and Fpn may play a key role in the improvement effects of

Yigong

Powder on ACD and iron metabolism.

关键词

运脾法拆方研究异功散慢性病贫血铁代谢小鼠

Keywords

activating spleen methodformula components analysisYigong Powderanemia of chronic diseaseiron metabolismmice

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